The overarching goal for Assay Development focuses on the timely delivery of robust and well validated assays to HTS Robotics. Our ability to clone, express and purify proteins, as well as to stably or transiently transfect cells from our own cell bank gives us the ability to design and develop new assays from scratch. We are not restricted to development of HTS assays for mammalian targets. Utilizing our dedicated microbiology facility we also focus on the discovery of novel antibacterials, antiparasitics and antifungals.
In most cases, assays are first developed either in the 96- or 384-well plate format and further miniaturized to 1536-well plate format for HTS. Our expertise encompasses both biochemical and cell-based assays development, covering a large panel of popular readouts such as absorbance, luminescence, fluorescence, fluorescence polarization, FRET, QFRET, TRF, TR-FRET (HTRF, LANCE, Delfia), FLIPR and AlphaScreen/AlphaLISA, but also custom assay formats such as phenotype readout assays. The breadth of our assay development capability is demonstrated by the range of target classes and assay platforms we already addressed, viewable below.
Species
- Bacteria
- Insect
- Mammalian
- Parasites (C. elegans, P. falciparum)
- Yeast
Detection Technologies
- Absorbance
- AlphaScreen, AlphaLISA
- Fluorescence Resonance Energy Transfer (FRET)
- Fluorescence Intensity (FLINT)
- Fluorescence Polarization (FP)
- High-Content / Phenotype
- Luminescence
- Molecular Devices FLIPRtetra (Kinetic Cell-Based Screening)
- Quenched FRET (QFRET)
- Time-Resolved Fluorescence (TRF)
- Time-Resolved FRET (a.k.a. TR-FRET, Lance, HTRF, LanthaScreen, et al.)
Target Classes
- Cell Fusion
- Enzyme
- Oxidoreductases
- Transferases (Kinases, etc.)
- Hydrolases (Metalloproteinases, etc.)
- Isomerases
- Ligases
- GPCRs
- Ion Channels
- Microorganisms
- Nuclear Receptors
- Phage
- Primary Cells
- Proliferation / Viability
- Protein Misfolding
- Protein Translocation
- Protein-Protein Interactions
- Protein-RNA Interactions
- Receptor-Ligand Interactions
- Stem Cells
- Transcription Factors